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KMID : 0545120000100010086
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.86 ~ p.90
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Cloning, Sequencing,and Characterization of Enterotoxin Pathogenicity Islet from Bacteroides fragilis 419
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Rhie, Gi Eun
Chung, Gyung Tae/Lee, Yong Jin/Sung, Won Keung/Oh, Hee Bok
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Abstract
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We have earlier reported on the cloning and identification of bft-k from an enterotoxigenic strain of Bacteroides fragilis 419, which was isolated from the blood of a Korean patient who suffered from systemic infections [4, 5]. The bft-k gene encodes a 397-amino-acids metalloprotease enterotoxin, and the protein has been identified as a new isoform of B. fragilis enterotoxins (BFTs), which are cytopathic to intestinal epithelial cells to induce fluid secretion and tissue damage in ligated intestinal loops [4, 6, 18, 20]. This report describes the cloning and sequencing of the enterotoxin pathogenicity islet of B. fragilis 419 which contains the bft-k gene. The cloned enterotoxin pathogenicity islet was found to have 6,045bp in length and to contain 12-bp direct repeats near its end. In the pathogenicity islet, in addition to the BFT-K, two putative open reading frames (ORFs) were identified; (1) the t-3 gene encoding a 396-aminoacids protein of a putative metalloprotease; (2) the third gene encoding an ORF of a 59-amino-acids protein, whose function has not yet been characterized. The expression of the t-3 gene in B. fragilis 419 was verified by western blot analysis.
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